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Background
Von Willebrand Factor (vWF) is a large protein that plays a key role in coagulation and wound closure. It is secreted by endothelial cells (EC) into the blood stream, a process that is influenced among other factors by the strain and shear stress acting on the ECs. Patients with unphysiological blood flow conditions (e.g. through circulatory support pumps) often suffer from disrupted levels of vWF. Current studies suggest that impaired secretion of vWF could play a role. A better description of the correlation between mechanical stimuli on ECs and the secretion of vWF would help to understand underlying mechanisms and allow for new treatments. A detailed study of these effects in vitro requires a bioreactor to contain the cells while applying controlled levels of flow and stretch.

Project Goal
In this project, a bioreactor is to be developed which allows the culture of endothelial cells under flow and stretch. The envisioned system should be capable of covering a range of flow rates and strains.

The project presents the opportunity of experiencing and driving the complete development process, including the design of the bioreactor and its manufacturing. Implementing the system into an existing flow loop and investigating interactions between unsteady flow rates and strains will complete the project.

In this project, you will:

• experience the complete development process of a bioreactor
• gain insights into cell culture and bio-engineering lab work
• benefit from an interdisciplinary work environment

 You bring:

• basic experience with CAD
• basic knowledge of fluid dynamics
• interest for experimental work
• ability to work independently and reliably
• systematic and methodical approach to problem solving

References:

[1] Zheng, W. ,et al. (2012). Lab Chip, 12, 3441-3450
[2] Turner, N. A., et al. (2009). Blood, 114(24), 5102–5111.