People

Victoria Makrides

Academic Guest University of Zürich
Institute of Physiology
Winterthurerstr. 190, Y23 J 40
8057 Zürich +41 (0) 44 635 50 37makrides@access.uzh.ch

Short Biography

Dr. Victoria Makrides joined the The Interface Group as an Academic Guest in September 2019. She provides a deep knowledge and expertise for projects in the fields of molecular and vascular neurobiology, human physiology, and cellular, animal and biochemical models. Dr. Makrides earned a PhD in Molecular, Cellular and Developmental Biology from the University of California, Santa Barbara with a specialization in Developmental Molecular Neuroscience.

Dr. Makrides began her career managing the Center for Clinical Pharmacology of Anti-microbials laboratory (Dr. Neil Benowitz, PI, Clinical Pharmacology and Therapeutics Division, UCSF), which provided analytical support for clinical research on AIDS and other infectious diseases. Subsequently, at UCSB, her doctoral and postdoctoral work in groups of Dr. Stuart Feinstein and Dr. John Lew focused on Tau, an Alzheimer’s disease (AD) and frontotemporal dementia with parkinsonism linked to chromosome 17 (FTDP-17) associated protein. As a senior research scientist in the group of Prof. Dr. med. Francois Verrey at the Institute of Physiology, she led a team investigating blood-brain barrier (BBB) transporter regulation of CNS interstitial fluid (ISF) homeostasis. She also collaborated with the Interface group in developing a robust systems biology method for identifying the activities of specific SLC transporters in cell culture models.  Dr. Makrides continues to be committed to investigating the roles and functions of the Neurovascular Unit particularly as it impacts developmental neurological pathologies, mental illnesses, and age-related dementias.

 

Book Chapters

  1. Makrides V*, Dolgodilina E, Virginito D. “Blood-brain Barrier Transporters and Neuroinflammation: Partners in Neuroprotection and in Pathology.” The Blood-brain Barrier and Inflammation, R Lyck and G Enzmann (eds.), Progress in Inflammation, Springer, scheduled for publication on May 7, 2017. (*corresponding author).
  2. Camargo SMR*, Makrides V*, Kleta R, Verrey F. Kidney Transport of Amino Acids and Oligopeptides and Aminoacidurias. Seldin and Giebisch’s The Kidney: Physiology and Pathophysiology, 2013; 2: 2405–2423. (*equal contribution).
  3. Verrey F, Ristic Z, Romeo E, Ramadan T, Makrides V, Dave MH, Wagner CA, Camargo SM. Novel renal amino acid transporters. Annu Rev Physiol. 2005; 67:557–72.

Peer Reviewed Journal Publications  

  1. Taslimifar T, Oparija L, Verrey F, Kurtcuoglu V, Olgac U*, Makrides V*. Quantifying the relative contributions of different solute carriers to aggregate substrate transport. Sci Rep. 2017 Jan 16;7:40628. Epub 2017 Jan 16. (*equal contribution).
  2. Dolgodilina E, Imobersteg S, Laczko E, Welt T, Verrey F*, Makrides V*. Brain interstitial fluid glutamine homeostasis is controlled by blood-brain barrier SLC7A5/LAT1 amino acid transporter. 2016 Nov;36(11):1929-1941. (*equal contribution)
  3. MakridesV*, CamargoSMR*, Verrey F. Kidney Transport of Amino Acids in the Kidney. Comprehensive Physiology. 2014; 4(1): 367-403. (*equal contribution).
  4. Pfeiffer F, Schäfer J, Lyck R, Makrides V, Brunner S, Schaeren-Wiemers N, Deutsch U, Engelhardt. Claudin-1 induced sealing of blood-brain barrier tight junctions ameliorates chronic experimental autoimmune encephalomyelitis. Acta Neuropathol. 2011 Nov;122(5):601-14. Epub 2011 Oct 9.
  5. Ruderisch N, Virgintino D, Makrides V*, Verrey F*. 2011. Differential axial localization along the mouse brain vascular tree of luminal sodium-dependent glutamine transporters Snat1 and Snat3. 2011 Jul;31(7):1637-47. Epub 2011 Mar 3 (*equal contribution).
  6. Lyck R, Ruderisch N, Moll AG, Steiner O, Cohen CD, Engelhardt B, Makrides V*, Verrey F*. 2009. Culture-induced changes in blood-brain barrier transcriptome: implications for amino-acid transporters in vivo. 2009 Sep;29(9):1491-502. Epub 2009 Jun 3 (*equal contribution)
  7. Camargo S M, Singer D, Makrides V, Huggel K, Pos KM, Wagner CA, Kuba K, Danilczyk U, Skovby F, Kleta R, Penninger JM, Verrey F. 2009. Tissue-specific amino acid transporter partners ACE2 and collectrin differentially interact with hartnup mutations. 136: 872-82. Epub 2008 Oct 29.
  8. Makrides V, Weber W, Bauer R, Wester HJ, Fischer S, Hinz R, Huggel K, Opfermann T, Herzau M, Ganapathy V, Verrey F, Brust 2007. Preferred Blood-Brain Transfer of O-(2-[18F]fluoroethyl)-D-tyrosine (D-FET) Compared to [18F]L-FET and 3-O-methyl-6-[18F]fluoro-L-DOPA. Brain Res.1147:25-33. Epub 2007 Feb 9.
  9. Danilczyk U, Sarao R, Remy C, Benabbas C, Stange G, Richter A, Arya S, Pospisilik JA, Singer D, Camargo SM, Makrides V, Ramadan T, Verrey F, Wagner CA, Penninger JM. Essential role for collectrin in renal amino acid transport. Nature, 444(7122):1088-91. Epub 2006 Dec 13.
  10. Ristic Z, Camargo SM, Romeo E, Bodoy S, Betran J, Palacin M, Makrides V, Furrer EM, Verrey F. Neutral Amino Acid Transport Mediated by Ortholog of Imino Acid Transporter SIT1/SLC6A20 in opossum kidney cells.. Am J Physiol Renal Physiol. 290(4):F880-7. Epub 2005 Oct 18.
  11. Makrides V*, Camargo SM*, Virkki LV, Forster IC, Verrey F. Steady-state Kinetic Characterization of the Mouse B0AT1 Sodium-dependent Neutral Amino Acid Transporter. Pflugers Arch. 451(2):338-48. Epub 2005 Aug 26. (*equal contribution).
  12. Verrey F, Ristic Z, Romeo E, Ramadam T, Makrides V, Dave MH, Wagner CA, Camargo SM. Novel Renal Amino Acid Transporters. Annu Rev Physiol. Sep 28.
  13. Ross JL, Santangelo CD, Makrides V, and. Fygenson DK. 2004. Tau Induces Cooperative Taxol Binding to Microtubules. PNAS;101(35):12910-5. Epub 2004 Aug 23.
  14. Makrides V, Massie M, Feinstein SC, and Lew J. 2004. Evidence for Two Distinct Binding Sites for Tau on Microtubules PNAS, 101(17):6746-51. Epub 2004 Apr 19.
  15. Makrides V, Shen TE, Bhatia RK, Smith BL, Thimm J, Lal R, and Feinstein SC. 2003. Microtubule Dependent Oligomerization of Tau: Implications for Physiological Tau Function and for Tauopathies. J Biol Chem. 29;278(35):33298-304.
  16. Baxter GT, Radeke MJ, Kuo R.C, Makrides V, Hinkle B, Hoang R,. Medina-Selby A, Coit A., Valenzuela P., and Feinstein SC. 1997. Signal transduction mediated by the truncated trkB receptor isoforms, trkB.T1 and trkB.T2. J Neuroscience, 17, 2683.
  17. Lee BL, Safrin S, Makrides V, Gambertoglio JG. 1996. Zidovudine, trimethoprom, and dapsone pharmacokinetic interactions in patients with human immunodeficiency virus infection. Antimicrobial agents and Chemotherapy, 40, 1231.
  18. Guerra-Romera L, Tureen JH, Fournier MA, Makrides V, Tauber MG. 1993. Amino acids in cerebrospinal and brain interstitial fluid in experimental pneumococcal meningitis. Pediatric Research, 33, 510.

Conference Contributions

  1. Contributions of Blood Brain Barrier Endothelial Amino Acid Transporters to Brain Interstitial Fluid Homeostasis. Victoria Makrides. Invited seminar. Clinical Biochemistry Colloquium, University of Zurich Pediatric Hospital, October 2013.
  2. Blood-brain barrier endothelial amino acid transporters in the control of brain interstitial amino acid homeostasis. Elena Dolgodilina, Martin Faltys, Sandra Krummenacher, Sonja Zimmer, Tobias Welt, Babette Weksler, Ignacio-Andres Romero, Pierre-Olivier Couraud, Francois Verrey, Victoria Makrides. Oral presentation. 14th International Symposium “Signal Transduction in the Blood-Brain Barriers”. 2012
  3. Expression of selected transporter, tight junction and signaling protein genes by the human in vitro Blood Brain Barrier endothelial cell model, hCMEC/D3. Martin Faltys, Babette Weksler, Ignacio-Andres Romero, Pierre-Olivier Couraud, Francois Verrey, Victoria Makrides. Barriers of the CNS 2012 Gordon Conference
  4. HDL stimulates endothelial CAT-1 expression and L-arginine-uptake: a novel mechanism leading to endothelial-protective effects of HDL that is impaired in patients with coronary disease. C Besler, V Makrides, K Heinrich, M Riwanto, K Huggel, D Poehlmann, F Verrey, TF Luescher, U Landmesser. European Society of Cardiology Congress 2011; European Heart Journal 2011 v. 32, pg 937-8.
  5. Organizing committee: 13th International Blood-Brain Barrier Symposium Zürich 2010, Signalling at the blood-brain and blood-tumour barriers, September 2 – 4, 2010, Zürich, Switzerland.
  6. Applications of the D3 human BBB cell model differentiated in shear stress flow for probing transendothelial (AA) transport. Invited seminar. Hoffman La Roche, 2010. Makrides V
  7. Brain Microvascular Endothelial Amino Acid Transport in vivo and in vitro Makrides V, Ruderisch N, Lyck R, Moll A, Weksler B, Romero IA, Cohen CD, Couraud PO, Engelhardt B, Virgintino D, Verrey F. Barriers of the CNS 2010 Gordon Conference.
  8. Blood-Brain Barrier Amino Acid Transport by the Human in vitro model hCMEC/D3 cultured under Physiologic Shear Stress Flow. Makrides V, Ruderisch N, Weksler B, Ignacio-Anderes R, Couraud PO, Verrey F, 36th International Congress of Physiological Sciences (IUPS2009) Kyoto, Japan Abstract No: 01510.
  9. Human Blood-Brain Barrier LAT1 Amino Acid Transendothelial Transport Kinetics. Makrides V, Ruderisch N, Frei K, Couraud PO, Verrey F. Barriers of the CNS 2008 Gordon Conference – Best Poster
  10. Blood-Brain Barrier Amino Acid Transporters. Ruderisch N, Makrides V, Baldinger M, Stiener O, Lyck R, Verrey F. 11th International Symposium “Signal Transduction in the Blood-Brain Barriers”. 2008, Abstr
  11. Fluoroethyl tyrosine (FET) isomers as substrates for Blood-Brain barrier amino acid transporters. Makrides V, Ruderisch N, Huggel K, Romeo E, Fakitsas P, Frei K, Brust P, Verrey F. Oral presentation 10th International Symposium “Signal Transduction in the Blood-Brain Barriers”. 2007, Abstract P5.
  12. Blood-Brain Barrier Amino Acid Transporters. Ruderisch N, Makrides V, Baldinger M, Stiener O, Lyck R, Verrey F. 10th International Symposium “Signal Transduction in the Blood-Brain Barriers”. 2007, Abstr P7.
  13. Blood-Brain Barrier Amino Acid Transporters: Expression and Applications. Makrides V, Ruderisch N, Brust P, Verrey F. Oral presentation Transporters 2006, International Symposium on Membrane Transport and Transporters. 2006, Abstr 47, Acta Biomedica 2006;77 Suppl 3:3-88.
  14. Essential role for collectrin in renal amino acid transport. Danilczyk U, Benabbas C, Makrides V, Remy C, Stange G, Camargo SMR, Verrey F, Wagner CA and Josef M. Penninger JM. Transporters 2006, International Symposium on Membrane Transport and Transporters. 2006, Acta Biomedica 2006;77 Suppl 3:3-88., pg 937-8.
  15. Diffusion of Ligands and Inert Polymers inside the Microtubule Lumen. Ross JL, MakridesV, and Fygenson DK. American Society for Cell Bology. Abstr 2003, 43. 325.1868.
  16. Microtubule Dependent Oligomerization of Tau: Implications for Tauopathies. Makrides V, Shen TE, Lal R, and Feinstein SC. Neuroscience. Abstr, 2001, 27, 542.4.